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The crystal datasheet says CL = 12.5 pF. But your PCB adds 3–5 pF of stray capacitance — from traces, pads, and the oscillator pin itself. If you don't subtract the stray from your external caps, the effective CL shifts and so does the frequency. Start with the formula in our Crystal Matching Guide, then verify on the bench.
The oscillator circuit needs enough gain to overcome the crystal's ESR, with margin. A common target: |R(neg)| ≥ 5× ESR at your highest operating temperature. Too little margin and the oscillator may fail to start at cold or low voltage.
Too much drive ages the crystal. Too little and it won't start. Tuning-fork crystals (32.768 kHz) are especially sensitive — often rated for <1 µW. If your oscillator stage drives harder than expected, add a series resistor. Check the crystal's max drive spec against your IC's oscillator output.